Physio ex 9 0 physical exercise 12 activity 1

Exercise doze: Serological Testing: Activity you: Using Direct Fluorescent Antibody Technique to Evaluation for Chlamydia Lab Report Pre-lab Questions Results You scored totally by responding to 4 away of 5 questions correctly. 1 . Which usually of the following has an epitope? You effectively answered: n. an antigen

2 . Chlamydia trachomatis can be

You correctly solved: c.

a bacterium that reproduces inside its web host cell.

several. The contagious form of Chlamydia that is propagate from person to person is a You properly answered: m. elementary physique that spots green from this activity

5. The goal of the direct neon antibody test is to check for the presence of You properly answered: c. antigen.

Experiment Results

Quit & Think Questions:

The source in the patient selections is

You effectively answered: c. epithelial scrapings

What is the identity for the precise part of the antigen that the antibody binds to? You properly answered: w. epitope

12-15. Drag the first slip (patient A) to the neon microscope. Rely the number of fundamental bodies the truth is through the microscope (recall that elementary systems stain green), enter the number of elementary systems in the field below, and then click Submit Info to display the results in the grid.

After you simply click Submit Data, the glide will automatically be placed in the biohazardous waste disposal. You answered: zero elementary physiques

16. Drag the next glide (patient B) to the neon microscope. Rely the number of elementary bodies you observe through the microscope (recall that elementary body stain green), enter the volume of elementary systems in the field below, and then just click Submit Info to display your results in the grid. As soon as you click Send Data, the slide will certainly automatically be placed in the biohazardous garbage disposal. You answered: 15 fundamental bodies

17. Drag the next slide (patient C) to the fluorescent microscopic lense. Count the number of elementary physiques you see through the microscope (recall that fundamental bodies spot green), your number of general bodies in the field below, and then click Submit Data to display your results in the main grid. After you click Submit Info, the slip will instantly be put into the biohazardous waste disposal. You answered: one particular elementary body

18. Drag the next glide (positive control) to the fluorescent microscope. Rely the number of fundamental bodies the truth is through the microscope (recall that elementary systems stain green), enter the range of elementary physiques in the field listed below, and then click Submit Info to display the results in the grid. Once you click Fill in Data, the slide will certainly automatically always be placed in the biohazardous waste disposal. You solved: 19 elementary bodies

19. Drag the final slide (negative control) towards the fluorescent microscopic lense. Count the quantity of elementary systems you see through the microscope (recall that elementary bodies discoloration green), enter the number of general bodies during a call below, then click Submit Data to show off your leads to the main grid. After you just click Submit Data, the slip will instantly be placed in the biohazardous waste disposal. You answered: zero elementary systems

Post-lab Questions Results

You won 100% by simply answering 5 out of 5 inquiries correctly. 1 ) In the serology test simply performed, the fluorescence is found on the You correctly solved: c. antibody

2 . What could most likely happen if you forgot to fix the samples to the slide? You correctly answered: a. They would all test out negative since the washing methods would remove the samples. a few. Which sample showed several residual nonspecific binding following the washing actions? You effectively answered: c. patient C

4. Which will patient samples contained the Chlamydia-specific antigen? You

correctly solved: b. affected person B

5. Just how is chlamydia disease transmission prevented?

You correctly answered: g. abstinence or faithful, common monogamy

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Review Sheet Results

1 . Describe the importance from the washing stages in the direct antibody fluorescence test. Your answer:

needed in order to remove virtually any non specific binding which may have occurred. 2 . Explain where epitope (antigenic determinant) is located. Your solution:

Upon antigens because they are large and possess multiple antigenic sites”locations that could bind to antibodies. three or more. Describe how a positive result is recognized in this serological test. Your answer:

If there is a presence of 10 or maybe more elementary physiques in a field diameter of 5 milimeters. They will discoloration green in red web host cells.

4. Just how would the results be affected if the negative control gave an optimistic result? Your answer:

It would be a “false negative and it will invalidate my own results.

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